Hello I have somewhat of a complex study that are I am trying to analyze.
I have 2 known cell line trx (Death) and wte (Lived) based on treatments that were used on them (0 = no treatment and 1=treatment). In addition, the cells were looked at for cell death via a type of staining (cross or byroll). What I would like to do is predict the fate of some other cell lines (tun, op7, t5h and ju8) by using the information that is already known about trx and wte. My interpretation is that, I can do this using a regression model such as a linear model to see which unknown cell lines closely matches the known cells or maybe use some type of agreement test like kappaetc to see which ones matches will but I need recommendations on other best tests that can help me answer this question.
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I have 2 known cell line trx (Death) and wte (Lived) based on treatments that were used on them (0 = no treatment and 1=treatment). In addition, the cells were looked at for cell death via a type of staining (cross or byroll). What I would like to do is predict the fate of some other cell lines (tun, op7, t5h and ju8) by using the information that is already known about trx and wte. My interpretation is that, I can do this using a regression model such as a linear model to see which unknown cell lines closely matches the known cells or maybe use some type of agreement test like kappaetc to see which ones matches will but I need recommendations on other best tests that can help me answer this question.
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Code:
* Example generated by -dataex-. For more info, type help dataex clear input byte replicate str6 Stain byte treatment int trx byte wte int(tun op7) byte t5h int ju8 7 "CROSS" 0 4934 18 3470 198 42 231 7 "CROSS" 0 4172 24 2690 179 41 151 7 "CROSS" 0 3667 24 2750 179 40 151 8 "CROSS" 0 8129 21 2750 184 38 146 8 "CROSS" 0 5079 24 2280 170 34 119 8 "CROSS" 0 4589 35 2020 165 32 85 8 "CROSS" 0 3984 30 2120 163 35 98 8 "CROSS" 0 6486 26 2520 182 38 119 9 "CROSS" 0 3829 22 2580 169 39 140 9 "CROSS" 0 3748 31 2200 165 35 97 9 "CROSS" 0 4389 28 1800 147 33 98 9 "CROSS" 0 3299 29 2110 163 34 231 10 "CROSS" 0 4187 21 2650 179 43 140 10 "CROSS" 0 3895 26 1830 142 34 79 10 "CROSS" 0 5799 25 2240 172 36 107 10 "CROSS" 0 4195 24 2730 180 40 151 10 "CROSS" 0 4453 26 2230 170 34 304 11 "CROSS" 0 4099 22 2930 186 40 121 11 "CROSS" 0 6295 23 2070 174 36 97 11 "CROSS" 0 4749 17 3350 173 40 258 11 "CROSS" 0 4647 28 3260 170 37 156 11 "CROSS" 0 5719 18 2670 175 36 134 11 "CROSS" 0 3798 35 2050 164 36 97 11 "CROSS" 0 4425 34 1800 157 37 86 11 "CROSS" 1 3995 30 1980 154 33 86 12 "CROSS" 0 9735 25 2650 177 34 121 12 "CROSS" 0 7140 23 2160 172 36 97 12 "CROSS" 0 3799 22 2640 168 35 121 13 "CROSS" 1 4424 19 3420 203 43 231 13 "CROSS" 1 3955 19 3430 197 43 250 13 "CROSS" 0 15906 21 4290 204 45 350 13 "CROSS" 1 4082 19 3400 200 42 231 14 "CROSS" 0 11995 17 3170 193 37 163 14 "CROSS" 0 5899 18 2410 174 36 134 14 "CROSS" 1 12990 14 3420 192 38 163 14 "CROSS" 1 4181 19 3370 200 43 231 15 "CROSS" 0 9690 17 2830 189 37 131 15 "CROSS" 1 13466 14 3830 201 41 302 15 "CROSS" 0 4697 25 1930 155 35 89 15 "CROSS" 0 4516 18 3370 198 41 250 15 "CROSS" 0 5397 41 2040 155 35 90 16 "BIROLL" 0 4296 21 2130 161 36 105 16 "BIROLL" 1 4060 18 3330 201 44 225 16 "BIROLL" 0 5222 19 3210 201 45 231 16 "BIROLL" 1 5379 14 4060 221 48 302 16 "BIROLL" 0 6303 14 4130 217 45 302 16 "BIROLL" 0 5104 22 3220 200 41 200 16 "BIROLL" 0 6850 25 1990 156 36 97 16 "BIROLL" 1 4816 20 3250 196 40 196 16 "BIROLL" 1 14500 14 3900 204 43 350 16 "BIROLL" 1 4733 19 3300 198 42 231 16 "BIROLL" 0 5172 19 3310 198 42 231 16 "BIROLL" 0 5189 20 3280 200 42 196 17 "BIROLL" 1 4482 25 2200 165 36 105 17 "BIROLL" 1 10371 16 4030 206 43 350 17 "BIROLL" 1 4504 22 3180 193 31 200 17 "BIROLL" 1 4723 19 3200 199 40 231 17 "BIROLL" 1 5886 16 3600 206 46 318 17 "BIROLL" 1 3291 20 2830 195 43 140 17 "BIROLL" 1 10372 16 3880 207 43 231 17 "BIROLL" 1 4010 18 3600 206 46 318 18 "BIROLL" 0 13594 12 4720 230 48 400 20 "BIROLL" 1 8814 21 4060 220 43 350 20 "BIROLL" 1 11385 14 4330 221 44 425 20 "BIROLL" 1 5705 16 3690 212 43 250 20 "BIROLL" 1 5798 18 3700 214 42 231 20 "BIROLL" 1 4890 18 3690 218 42 231 20 "BIROLL" 1 7827 15 4080 222 43 350 21 "BIROLL" 1 6342 17 3740 220 46 225 21 "BIROLL" 1 5788 18 3670 218 43 231 22 "BIROLL" 1 11497 12 4840 233 51 400 23 "BIROLL" 1 6165 15 3720 212 44 302 end
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Code:
encode stain, gen(stain1) bysort treatment stain1: regress trx ju8 end
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encode stain, gen(stain1) bysort treatment stain1: kappaetc trx ju8 end